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Growth Hormone, IGF-1, MGF, Insulin and Peptides Discuss here everything related to HGH, IGF-1, MGF, Insulin and Peptides |
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Research Acetic Acid .6% don't throw away your frag I think you will be O.K. brotha!!!
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Disclaimer: I do not promote the use of anabolic steroids without a doctor's prescription. The information we/I share is for entertainment purposes only. I do not sell, or condone the use of anabolic steroid. |
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Acetic Acid Conv. for research only
0.6% Acetic Acid Overview
Acetic Acid (AA) will be used to reconstitute (turn your lyophilized igf-1 into a liquid form) your igf-1. The standard is to use 0.6% AA. This concentration is typically not available for you to purchase. You can make your own 0.6% AA and I will show you how below (many have used this method successfully). Making 0.6% Acetic Acid You will have to purchase a few items upfront. Here is a ***8220;grocery list***8221; of items you will need. I have provided check boxes for you to check off once you have purchased these items. Items Needed: ***8226; Distilled white vinegar (grocery store) ***8226; Distilled water (grocery store) ***8226; 0.2-0.22um sterile Whatman syringe filter ***8226; 10mL syringe with a luer lock tip ***8226; ~20-22 gauge needles (just the needles) ***8226; Sterile glass vial (10-20mL) ***8226; Alcohol prep pads ***8211; sterile kind (70% isopropyl alcohol) Quick Guide: 1. Swab the top of your sterile vial with alcohol prep pad (70% isopropyl alcohol) 2. Mix 7.5mL distilled water with 1.0mL vinegar 3. Add Whatman syringe filter 4. Add sterile ~20ga. needle to end of Whatman filter 5. Inject the 8.5mL of solution into the sterile vial 6. You now have sterile 0.6% acetic acid Detailed Directions: 1. Wash you hands thoroughly 2. Optional: wear alcohol treated exam gloves (rub your gloved hands together with 70% isopropyl alcohol on them until dry) 3. Using a sterile alcohol prep pad, swab the top of your sterile glass vial (into which the acetic acid solution will be held in) 4. Using the 10mL syringe with a ~20ga. needle on the end, draw up 7.5mL distilled water 5. Using the same syringe, now draw up 1mL vinegar 6. Remove needle from the syringe and discard 7. Attach 0.2-0.22um Whatman sterile syringe filter (do not touch the free end that will have a needle on it) 8. Put a new, sterile needle (~20 gauge) onto the free end of the Whatman filter (do not touch needle) a. Do not use the same needle on the Whatman that was used to originally draw up the unsterile vinegar and distilled water. 9. Put a ~20 gauge sterile needle into the top of your sterile glass vial to act as a vent 10. Inject the acetic acid solution into the vial 11. You are now done and should have sterile 0.6% acetic acid Notes: 1. These items MUST be sterile: 20-22ga. Needles, whatman filter, glass vial 2. Whatman filter: These small, sterile filters are used to filter the acetic acid solution so it is sterile. It does not matter that the liquid in your syringe (distilled water & vinegar) is not sterile, nor does it matter that the syringe itself is not sterile. Once the liquid goes through the filter it is STERILE. Thus, everything after the filter must be sterile! 3. You will most likely use 1mL (milliliter) of 0.6% AA to reconstitute your igf-1. Thus, you should make at least 1.5mL. In reality, it***8217;s just as easy to make 8.5mL as I have stated in the above directions. You will have plenty for use later then. 4. Do NOT reuse the Whatman filter nor any needles! Discard immediately.
__________________
Disclaimer: I do not promote the use of anabolic steroids without a doctor's prescription. The information we/I share is for entertainment purposes only. I do not sell, or condone the use of anabolic steroid. |
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Quote:
__________________
Disclaimer: I do not promote the use of anabolic steroids without a doctor's prescription. The information we/I share is for entertainment purposes only. I do not sell, or condone the use of anabolic steroid. |
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Sir frag is the one peptide that is supposed to be cloudy. A quick google search wouldve explained this but let me elaborate. ( if there are flakes you just need to roll the vial back and forth for a moment )
The chemical sequence of HGH Fragment is such that it contains very hydrophobic amino acids. Hydrophobic amino acids are very difficult to dissolve. Hydrophobic molecules are literally "water- fearing". A good example of a hydrophobic compound is oil- petroleum. You could see this when BP's oil well spilled millions of gallons of oil into the Gulf. It was not "diluted" by the water. Water is actually an excellent solvent for most peptides. HGH Fragment is by far the most hydrophobic of any peptides available related to GH research. Now, you have probably purchased HGH Fragment in the past and not had this issue. for reasons I cannot fully explain- this is not an issue with every reconstituted vial. The process in Some manufacturing plants includes using a copious amount of acetonirile. To put this into perspective- when our peptides are being synthesized and then tested for purity- a small amount of acetonirile is used to remove the impurities from a column that is being tested during the HPLC- .1% aceto Now acetonirile is a poisonous substance and needs to be removed from the final product- (it metabolizes into cyanide). If this substance is not removed, the effects can be very dangerous indeed. Some chemists use 10% acetonirile to dissolve the HGH Fragment! This should not be the case. So, although this is very counter-intuitive, the fact that your HGH Fragment is cloudy tells you that it is true HGH Fragment, and that it does contain copious amounts of acetonirile. We know it is not aesthetically pleasing, and again it is hard to wrap our minds around the fact that if something is cloudy that it might not be "bad for us" as compared to something that is not. It is counter to our experiences in life. Here is the "fix". 1ml of .6% Acetic Acid will completely resolve the situation! The peptides are completely solvent in that mixture. |
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LinkBack to this Thread: https://www.hypermuscles.com/f62/hgh-fragment-176-191-a-1313/
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AOD-9604 revisited | BigFooty AFL Forum | This thread | Refback | 03-27-2014 04:36 PM |