Steroids Source Talk | Anabolic Steroid Forum - View Single Post - IGF-1 LR3 Overview

admin · #1 (**permalink**) 12-09-2015, 08:13 PM

This is a copy paste from other site. I hope it will be useful to some of you.
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Background:
Long Arg3 Insulin-like Growth Factor-I (Long-R3-IGF-I) is an 83 amino acid analog of IGF-I comprising the complete IGF-I sequence with the substitution of an Arg for the Glu at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus. Long-R3-IGF-I is significantly more potent than IGF-I in vitro. The enhanced potency is due to the markedly decreased binding of Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.

Description:
Recombinant Human Long-R3-IGF-I produced in E. coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9111 Dalton.

0.6% Acetic Acid Overview
Acetic Acid (AA) will be used to reconstitute (turn your lyophilized IGF-1 into a liquid form) your IGF-1. The standard is to use 0.6% AA. This concentration is typically not available for you to purchase. You can make your own 0.6% AA and I will show you how below (many have used this method successfully).

Making 0.6% Acetic Acid
You will have to purchase a few items upfront. Here is a ***8220;grocery list***8221; of items you will need. I have provided check boxes for you to check off once you have purchased these items.

Items Needed:
***8226; Distilled white vinegar (grocery store)
***8226; Distilled water (grocery store)
***8226; 0.2-0.22um sterile Whatman syringe filter
***8226; 10mL syringe with a luer lock tip
***8226; ~20-22 gauge needles (just the needles)
***8226; Sterile glass vial (10-20mL)
***8226; Alcohol prep pads ***8211; sterile kind (70% isopropyl alcohol)

Quick Guide:
1. Swab the top of your sterile vial with alcohol prep pad (70% isopropyl alcohol)
2. Mix 7.5mL distilled water with 1.0mL vinegar
3. Add Whatman syringe filter
4. Add sterile ~20ga. needle to end of Whatman filter
5. Inject the 8.5mL of solution into the sterile vial
6. You now have sterile 0.6% acetic acid

Detailed Directions:
1. Wash you hands thoroughly
2. Optional: wear alcohol treated exam gloves (rub your gloved hands together with 70% isopropyl alcohol on them until dry)
3. Using a sterile alcohol prep pad, swab the top of your sterile glass vial (into which the acetic acid solution will be held in)
4. Using the 10mL syringe with a ~20ga. needle on the end, draw up 7.5mL distilled water
5. Using the same syringe, now draw up 1mL vinegar
6. Remove needle from the syringe and discard
7. Attach 0.2-0.22um Whatman sterile syringe filter (do not touch the free end that will have a needle on it)
8. Put a new, sterile needle (~20 gauge) onto the free end of the Whatman filter (do not touch needle)
a. Do not use the same needle on the Whatman that was used to originally draw up the unsterile vinegar and distilled water.
9. Put a ~20 gauge sterile needle into the top of your sterile glass vial to act as a vent
10. Inject the acetic acid solution into the vial
11. You are now done and should have sterile 0.6% acetic acid

Notes:
1. These items MUST be sterile: 20-22ga. Needles, whatman filter, glass vial
2. Whatman filter: These small, sterile filters are used to filter the acetic acid solution so it is sterile. It does not matter that the liquid in your syringe (distilled water & vinegar) is not sterile, nor does it matter that the syringe itself is not sterile. Once the liquid goes through the filter it is STERILE. Thus, everything after the filter must be sterile!
3. You will most likely use 1mL (milliliter) of 0.6% AA to reconstitute your IGF-1. Thus, you should make at least 1.5mL. In reality, it***8217;s just as easy to make 8.5mL as I have stated in the above directions. You will have plenty for use later then.
4. Do NOT reuse the Whatman filter nor any needles! Discard immediately.